FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13960349
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT411070.2944553893315991No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT289730.20753779149790597No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA235250.16851297915259855No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC230750.16528956403597073No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC179680.12870738403459686No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG147970.10599305217942619No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG147610.10573517897009596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT30500.053.8742031
CGCAAAA80650.051.0527651
ACCGTTG35150.046.342683
TAACCCC91050.044.3939939
GACCGTT38450.042.4883422
ACCCGGC71950.040.39685
CACCCGG77400.037.6126064
TTAACCC110650.036.4868058
CCGTTGA45750.035.6054884
TCACCCG89050.033.9184153
CGGCCCG98550.030.1707468
CTCGATT61050.028.31656348-49
ATTCCGT61600.027.8698952-53
GCAAAAT149550.027.500412
CGTTCTT64150.026.99143876-77
CCAGTAG56050.026.8602455
GTAGCAT59050.025.9002728
CGTTGAC65550.025.5757485
CGGACAC70050.025.42947612-13
ATCGACC87900.025.31397442-43
Produced by FastQC (version 0.11.5)