FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13882751
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT407330.2934072648857564No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT288440.20776861877015587No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA232700.16761807512070193No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC227740.16404529620966335No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC180070.12970772147393556No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG148640.10706811639854379No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG147000.10588679433924876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT29800.057.536711
CGCAAAA83950.052.332931
ACCGTTG34850.049.2015533
TAACCCC93950.046.2617659
GACCGTT38700.044.5506862
ACCCGGC72800.041.6917535
CACCCGG79050.038.635374
TTAACCC113950.038.2251978
CCGTTGA46150.037.4632644
TCACCCG91100.034.046243
CGGCCCG97050.031.4709728
GCAAAAT153000.028.9012762
CTCGATT62800.027.94349348-49
ATTCCGT62650.027.70717252-53
CCAGTAG53500.027.0786325
CGTTGAC64850.027.0269495
ATCGACC91250.025.65956742-43
ACGGACA61250.025.59605218-19
CGGACAC69500.025.42635712-13
CCGCATG92750.025.37310670-71
Produced by FastQC (version 0.11.5)