FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13969927
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT411330.29443962019271824No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT291830.208898729392072No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA237020.16966445136041153No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC229240.1640953456664448No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC179090.12819680446433257No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG151390.10836849755907815No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG148130.10603491342510236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT30050.061.7983471
ACCGTTG35900.051.19953
CGCAAAA84650.049.4303321
GACCGTT39350.046.951832
TAACCCC92300.044.358639
ACCCGGC72600.041.3466725
CCGTTGA46900.039.5963064
CACCCGG77250.038.9184954
TTAACCC110700.037.199968
TCACCCG91050.034.062963
CGGCCCG95750.031.4500588
CTCGATT62250.028.60938348-49
CCAGTAG52800.027.9760635
ATTCCGT62600.027.88043852-53
GCAAAAT153600.027.7054022
CGTTGAC68800.027.1999385
CATTCGG33000.026.1943864
ATCGACC86100.025.59373942-43
AATTAAC166950.025.3771726
CGTTCTT69000.025.3705276-77
Produced by FastQC (version 0.11.5)