FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13010049
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA199120.15305092240621077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG24050.029.943621
CCCGTAT29950.028.9812411
GTATCGA27250.028.0571464
TATCGAA32100.023.965925
GTTCGAC17800.020.54288728-29
TCGACCG18400.020.26108230-31
CGTATCG56550.020.0700933
CCGTATC58800.019.3021052
CGATCCG19700.018.808882-83
ACGACTT25400.018.7762431
CGCGTAT2304.1313637E-516.5884021
ACGTTCG11000.016.4049556
TGTCGAG14500.015.7201755
CTCGCGT7500.015.261331
CTCCGGT29750.015.2292681
ATCGCGT15150.015.20258294-95
CGTGCCG107200.015.0259568-69
CGACCGT20900.014.88418432-33
CGTACGC43800.014.745145
CCGTCGG48450.014.7021752
Produced by FastQC (version 0.11.5)