FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13010049
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT353810.27195132009110806No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT279240.2146340878500919No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG187400.144042501300341No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA167000.12836231439251305No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC157090.12074512555640643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT22600.053.1689341
ACCGTTG26450.046.1499943
ACCCGGC81700.044.6493455
CGCAAAA68900.041.8422781
TCACCCG88050.041.7518583
TAACCCC68700.040.9325949
CACCCGG90550.040.337644
GACCGTT30550.039.799532
CGGCCCG103750.035.436478
CCAGTAG55450.032.7218445
TTAACCC87900.032.0992748
GTAGCAT59450.031.4006258
TAGCATA62000.030.1096129
CTCGATT71350.030.05266248-49
CCGTTGA40400.029.9799824
ATTCCGT72250.029.38250552-53
CGTTCTT77300.027.28361776-77
CGGACAC81250.026.80733712-13
ATAGCTC82850.026.22574238-39
CAGTAGC69850.026.1806416
Produced by FastQC (version 0.11.5)