FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13089985
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA195830.14960292162290484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT27750.033.6840441
GTATCGA24400.033.4762044
CCCGTCG25900.032.0391271
TATCGAA30800.027.291045
CGTATCG55300.022.1560383
CCGTATC60300.020.3188882
TCGACCG17450.019.59481830-31
TGTCGAG12200.019.462915
ATCGCGT15850.019.0268594-95
TCGCGTG2603.2941898E-718.265192
GTTCGAC18500.018.22590628-29
GTTACGA9750.017.5345844
TTACGAC10400.017.351935
TGTTACG9650.017.224173
ACGCTTA20450.016.95272644-45
CGATCCG20150.016.8559782-83
ACGACTT24150.016.5879731
CCGTCGG50450.016.2848032
CCCGGTT18500.015.9827361
CGGCATG49750.015.8457416
Produced by FastQC (version 0.11.5)