FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13869316
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA343270.24750319338026475No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG279830.20176193259999267No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT190060.13703631815729053No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA164050.11828268964381516No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA153940.11099321696902717No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT144060.10386957799505038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT71800.042.846411
GTATCGA72900.040.9744
CCCGTCG33250.040.164791
TATCGAA78050.038.5137675
CCGTATC126600.029.183082
CGTATCG128150.029.0895023
TGTCGAG10250.028.261245
ATCGAAG126250.026.8566656
ACGACTT21900.026.3524131
CGAAGGC122550.024.8000138
TCGAAGG142250.023.835887
TCGACCG21400.022.52358630-31
GAAGGCC137000.022.357569
GTTCGAC21900.022.1177728-29
CGGCATG61200.020.562686
ACGTTCG16400.020.2693336
GTCGAGG15100.019.8129376
TCAATCG50100.019.8104278
CGATCCG24200.019.72711682-83
AAGTTCG25000.019.47014226-27
Produced by FastQC (version 0.11.5)