FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13869316
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT601650.43379933083938677No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC480590.3465131229254565No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT393990.28407312948958696No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA365520.26354580139352224No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC333320.2403290832799541No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG310870.22414227204859993No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG294320.21220945575109834No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT236350.1704121529857709No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC235370.16970555721709707No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT224370.16177438022177879No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA218950.1578664730113583No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG212850.15346827485940906No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA205940.1484860536741682No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC197570.14245114899682146No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA197410.14233578642234412No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT189680.1367623320429068No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT188080.13560870629813324No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT183070.131996415684811No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT176740.12743238383205055No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA175200.12632201905270599No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC172340.12425991303392322No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT148200.10685458460965198No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC141410.10195888535526915No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA141200.10180747197626762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT34300.067.012551
ACCGTTG38300.060.2622833
CACCCGG112100.055.201394
GACCGTT42400.054.099012
ACCCGGC115500.053.4553685
CGCAAAA81300.048.716991
TCACCCG142150.044.2332843
CCAGTAG68800.043.765395
CGGCCCG146000.042.5198638
TAACCCC98800.039.7097329
TTAACCC98000.039.4033938
GTAGCAT79300.038.093698
CCGTTGA62000.037.150214
TAGCATA85200.036.4038549
CGCAAGT36150.036.2619486
CGGACAC105000.034.47308712-13
CTCACCC186400.033.375932
CTCGATT109250.033.25533748-49
ATTCCGT110050.032.77493752-53
GACACGG111650.032.59201414-15
Produced by FastQC (version 0.11.5)