FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13886404
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA331580.23878032066473076No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG272340.1961198882014379No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT187770.13521859222877283No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA161570.1163512166288695No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA151520.10911392178997528No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT141180.10166778958757068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT69100.041.485961
GTATCGA70550.040.051234
CCCGTCG31400.037.5207941
TATCGAA75700.037.263745
CCGTATC120300.028.7778132
CGTATCG120550.028.7181343
TGTCGAG12300.028.1847135
ACGACTT25050.027.2290841
ATCGAAG128650.025.2488846
CGAAGGC119150.023.9937618
TCGACCG24700.023.64869530-31
TCGAAGG145350.022.6746377
GTTCGAC25600.022.53895428-29
GTCGAGG16100.022.4173166
GAAGGCC132700.021.6869079
CGATCCG27800.020.93256682-83
AAGTTCG27950.020.72878826-27
ACGTTCG16750.019.846286
CGGCATG57350.019.2938296
CGCTAGG4700.019.2823721
Produced by FastQC (version 0.11.5)