FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13886404
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT599620.4318036548554975No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC481450.34670602986921595No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT389210.2802813456961212No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA365020.2628614290639967No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC334510.2408902981650253No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG312280.2248818340586951No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG295980.21314373397173236No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT241300.1737670890174303No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC235370.16949672499806284No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA223190.16072555573062688No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT222360.16012784879368339No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG213270.15358187764089248No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA205400.1479144636725246No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC200410.14432102076246664No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA198980.14329123652170858No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT190930.13749419936219628No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT188480.13572988370495342No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT181860.1309626307861992No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT180990.13033611869566808No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA175570.12643302038454304No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC173520.12495675626317657No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT146250.10531884280480389No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC142770.10281279444267932No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA141590.101963042411844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT37300.063.4104161
ACCGTTG43000.055.006433
CACCCGG114050.053.3885884
ACCCGGC115100.052.778715
GACCGTT45050.052.2925072
CGCAAAA85200.047.9958461
CCAGTAG66150.044.660555
TCACCCG143350.044.165693
CGGCCCG141550.043.1867948
TAACCCC98400.041.3683369
TTAACCC96150.041.2495778
GTAGCAT79200.037.8430828
CGCAAGT33450.036.9183546
CCGTTGA67350.035.613084
TAGCATA86400.034.8544169
CGGACAC103950.034.54778712-13
GACACGG110000.032.75727514-15
CTCACCC187300.032.7371642
CGGACAG109950.032.60016618-19
CTCGATT109050.032.57537548-49
Produced by FastQC (version 0.11.5)