FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13465267
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA245530.18234320938455953No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG222580.16529935871305038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG27200.029.9925441
GTATCGA21650.027.1990474
CCCGTAT26950.026.907351
TATCGAA29100.020.8884815
CGCGTTT4600.020.7423651
ACGACTT20700.019.820481
TCGACCG18700.019.6811330-31
CGTATCG61450.019.551883
GTTCGAC19250.019.24215928-29
CGATCCG20050.018.71610882-83
TCGACGA71750.018.63147444-45
CCGTATC66250.018.2786522
TAAGCCG76650.018.2257666-67
CCGTCGG43200.018.0281032
GACGAAA75700.017.78551546-47
CGCTAGT3007.324343E-817.4927271
ATCGCGT16900.017.42391294-95
TACGAGC17450.017.4170446
GTTACGA8750.017.3672814
TTACGAC8900.017.0745745
Produced by FastQC (version 0.11.5)