FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13465267
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT407670.30275671473874227No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT291020.21612642363497137No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT219210.1627966233421142No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG199820.14839661181616376No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT194460.14441600006891805No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA156460.1161952451444149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG88800.047.3842053
ACCCGGC96450.043.675945
CACCCGG105800.039.815524
CGACCGT14450.036.809081
CGGCCCG115300.036.086048
TAGCATA66550.033.330099
CCAGTAG66000.032.8846445
GTAGCAT70200.031.5968828
CTCGATT83000.029.32501848-49
CGGACAC86200.028.68290112-13
ATTCCGT85850.028.51703852-53
GTCAACC103650.028.5008686
CGTTCTT89600.026.87897976-77
GACACGG92950.026.80716514-15
CTCACCC161800.026.3870452
CCGGCCC163400.026.3639227
CGTGGGT93150.026.05287756-57
CGGACAG94250.025.9858418-19
ATAGCTC94350.025.94981638-39
TTCTCGA94150.025.92787446-47
Produced by FastQC (version 0.11.5)