FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13414039
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA242240.1805869209117403No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG216690.161539712237306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT28150.031.5111621
GTATCGA24750.028.588944
CCCGTCG26550.028.3806571
TATCGAA32250.022.9711045
CGTATCG63650.020.6665693
CCGTATC68050.019.5396562
TCGACGA74400.019.2798144-45
TTACGAC8450.019.1077355
CTTCGCG19800.018.7075276
GACGAAA76950.018.51742446-47
TAAGCCG77600.018.4306766-67
TTCGCGC19900.018.3748847
ACGACTT20900.018.2546521
TCGACCG16800.018.09180830-31
GTCCGTT12500.017.9315451
CCGTCGG41200.017.7504962
ATCGCGT18800.017.55579294-95
GTTACGA8150.017.4803714
CGAAACA81300.017.46805448-49
GTTCGAC18500.016.55705528-29
Produced by FastQC (version 0.11.5)