FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13414039
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT406310.3028990746187632No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT287560.21437241982075644No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT219750.16382090435252203No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG199220.1485160435272329No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT191120.14247759381048467No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA156380.11657935391420884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG92400.045.234853
ACCCGGC94500.043.979315
CGACCGT15800.039.6784821
CACCCGG107400.038.8736344
CGGCCCG115800.036.137038
CCAGTAG64800.033.350985
TAGCATA66300.032.955869
GTAGCAT69450.032.2131928
CTCGATT82750.030.50380748-49
GTCAACC107650.029.6505186
ATTCCGT85100.029.5498452-53
CGGACAC88850.028.76466612-13
GACCGTT22400.028.199632
ACCGTTG22900.027.7927673
CGTTCTT89800.027.71666176-77
CGGACAG92750.027.20002718-19
TTCTCGA93100.026.85761646-47
CTCACCC158700.026.8445012
GACACGG96450.026.6471414-15
CCGGCCC161550.026.6380927
Produced by FastQC (version 0.11.5)