FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13511673
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT408930.3026494202457386No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT297210.21996535884194354No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT222810.1649018593034334No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG200000.14802016004975846No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT197410.14610329897711408No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA158680.11743919498347836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG88700.045.4063223
ACCCGGC93050.043.1837735
CACCCGG104800.038.2950554
CGACCGT15200.037.8073461
CGGCCCG110450.036.1663678
TAGCATA63650.029.7003049
CCAGTAG63650.029.1772885
ACCGTTG19900.028.8786163
ATTCCGT83150.028.55757152-53
CTCGATT83450.028.36954348-49
GTAGCAT67300.028.1599868
CGGACAC85800.027.7937612-13
GACCGTT21100.027.4612242
GTCAACC105950.027.3910356
CGGACAG89600.026.75022718-19
CCGGCCC154450.026.5088427
CTCACCC154800.026.4778252
CGTTCTT89200.026.30884776-77
CGTGGGT91250.025.81451456-57
TTCTCGA93850.025.55469146-47
Produced by FastQC (version 0.11.5)