FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13367539
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA228850.17119830359200747No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG195630.14634705759975714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG31600.036.2270971
GTATCGA23100.036.182654
CCCGTAT26950.035.5751461
TATCGAA32500.026.1558825
CCGTCGG51450.021.598832
TCGACCG19750.021.03991330-31
CGTATCG68350.020.8440673
ACGACTT22700.020.8026521
TCGACGA65950.020.27086444-45
CCGTATC73000.019.7765462
GACGAAA67500.019.6651546-47
CGATCCG21500.019.66427882-83
GTTCGAC21250.019.55466828-29
TAAGCCG69250.019.3138866-67
ATCGCGT24900.018.6923194-95
CGAAACA71750.018.63304548-49
CGGCATG57950.018.2747386
ACGTTCG15200.017.1837866
CCGAGCT80100.016.72858870-71
CCCAAAT175400.016.6429861
Produced by FastQC (version 0.11.5)