FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13367539
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT504710.3775638881622115No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG235900.17647227361745493No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT212200.15874275736169538No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA173430.12973966262600767No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT173070.12947035351832525No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT141880.10613771166106192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG104850.050.237363
ACCCGGC105950.049.1789325
CGACCGT17100.046.10691
CACCCGG114350.045.7733154
CGGCCCG130500.040.1468478
CCAGTAG71500.034.410995
ACCGTTG23750.034.3976333
TAGCATA76150.033.495969
GTAGCAT75550.033.4474958
GACCGTT24300.032.8369982
CTCACCC163600.032.4287572
CTCGATT99100.030.6463448-49
CGGACAC101800.030.40157712-13
ATTCCGT100800.030.08248552-53
CGGACAG105600.029.01729818-19
TTCTCGA107650.028.27845446-47
CGTTCTT106950.028.13855776-77
GACACGG111100.027.87965614-15
CGTGGGT108400.027.84205256-57
CCGGCCC193000.027.7858097
Produced by FastQC (version 0.11.5)