FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14507155
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA159130.1096907008989702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT26950.033.804711
GTATCGA24150.028.512374
CCCGTCG25200.026.499041
TATCGAA33300.020.1074665
CGTATCG53500.019.6164743
TCGACCG15700.019.35810330-31
TCGACGA40200.018.60531244-45
ATCGCGT22850.018.49830494-95
CCGTATC61150.018.0943052
TAAGCCG43750.017.2695166-67
ACGACTT22950.017.2503571
CGATCCG17650.016.95603482-83
GACGAAA44500.016.59475546-47
GTTCGAC18000.016.48883628-29
CCCGTCT44100.016.1157441
CGACCGT16300.015.87783832-33
CCGTCGG42550.015.7362792
CGTACGC38200.015.2905895
CCGTACG38550.015.2749494
CGAAACA48800.015.22998348-49
Produced by FastQC (version 0.11.5)