FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14442230
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA154750.10715104246366385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT26350.031.1275711
GTATCGA23300.028.3299144
CCCGTCG25500.025.05891
CGTATCG52550.020.3327523
TATCGAA33600.019.504115
TCGACGA43700.018.91220344-45
CCGTATC59550.018.8996142
TCGACCG12800.018.5515230-31
ATCGCGT20750.017.39386794-95
TAAGCCG49050.017.00042766-67
GACGAAA48950.016.93213746-47
CCCGTCT43850.016.4211941
GTTCGAC15000.015.8296728-29
CGAAACA53750.015.42005848-49
CCCAAAT172300.015.3051391
ACCCGTT35500.015.2497552
CGATCCG16650.014.6932782-83
TGTCGAG16300.014.5669545
CCGTCGG46850.014.3934562
CGTACGC37500.014.1831645
Produced by FastQC (version 0.11.5)