FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14525968
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT289760.1994772396579698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG78000.037.753173
CGACCGT15600.036.230241
ACCCGGC83700.035.409885
CACCCGG86150.034.4023934
CGGCCCG96350.030.6139018
CTCGATT63400.027.75370248-49
ATTCCGT66950.026.14019852-53
GACCGTT21550.026.0069582
ACCGTTG22900.024.681293
CGTTCTT72400.023.88327876-77
TTCTCGA73600.023.87513746-47
CGGACAC76050.023.01769812-13
CGATTTG72650.022.95690294-95
CGGACAG76850.022.74866918-19
ACGGACA67600.022.66392318-19
CGTGGGT78250.022.2742856-57
GCGATTT67300.021.81650594-95
TAGCATA65100.021.6697659
TCGATTC70900.021.66955650-51
TCTCGAT71600.021.6566948-49
Produced by FastQC (version 0.11.5)