FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14013873
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT752910.5372604703924461No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA626880.44732815831854617No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT573030.40890195023174536No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC557540.3978486175805932No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG468020.3339690605159616No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC435410.3106992620812248No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG401820.2867301566098108No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG385160.2748419369862992No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC368330.26283240899928234No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA352940.2518504342090156No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA344630.24592059596943686No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT332640.2373647884492745No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT310620.22165178748230416No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT297080.21198993311841774No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT289840.206823623990313No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA272100.19416473946923882No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC267300.19073956214673846No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA244060.1741559952769659No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG239360.17080217581535098No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC238880.17045965808310096No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT236480.16874706942185078No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG233900.16690603661100684No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT233830.166856086108387No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC208000.148424350641682No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC202670.1446209766564889No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA201210.1435791518875617No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC194530.13881244678041538No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT187850.13404574167326905No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG184310.13151967339792506No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA181550.12955019643748736No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA177770.1268528692960183No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA171200.12216465783584594No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT170040.12133690664957503No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC166580.11886792466293936No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT147480.10523857323382338No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC146360.10443936519190662No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC141790.10117831094944274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT56700.065.74591
ACCGTTG64350.058.9641573
CGCAAAA98350.058.810521
GACCGTT63700.058.447162
CACCCGG131100.057.4504474
CCAGTAG90550.052.2371335
ACCCGGC146300.051.5807955
GTAGCAT106400.044.5075048
TCACCCG177550.043.8377383
TAGCATA111650.042.8414769
TTAACCC134350.042.7079778
TAACCCC140150.041.7891659
CGGCCCG186600.040.8548058
GCCAGTA116500.040.763084
CAGTAGC116600.040.4473576
CTCACCC194900.039.0822262
TGCCAGT122600.038.850833
GTTGACT169650.038.404521
CAAAATT158300.036.388973
ACATAAA100600.035.9707874
Produced by FastQC (version 0.11.5)