FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14016870
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA672200.479564981340342No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA354240.2527240389616227No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG327810.23386818883245689No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT225350.1607705571928683No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC218470.15586218606579072No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG198260.1414438458800003No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG185100.1320551592473926No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA184990.13197668238344223No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC183100.13062830717556773No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA174310.12435729231989737No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG173690.12391496817763167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG50650.039.9316751
CCCGTAT86100.037.2303541
GTATCGA87450.036.274834
ACGACTT32300.036.1821751
TGTCGAG14150.034.5676235
TATCGAA93050.034.244825
CGCTAGG4550.032.4998551
TGTTACG12100.031.004913
GTTACGA12500.030.392664
TTACGAC13100.029.000635
GTCGAGG17650.027.174746
TTCGCGC40250.027.0183187
GTCGGTA6650.026.5406281
CCGTATC167900.024.946332
CGTATCG171700.024.8920653
CTTCGCG45500.024.5270446
ATCGAAG155600.024.018946
TCGACCG39900.023.62634330-31
GTTCGAC40600.023.1596828-29
GAAGGCC143800.022.720529
Produced by FastQC (version 0.11.5)