FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14016870
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT752810.5370742540952438No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA623120.4445500314977595No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT575160.4103341188153989No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC553630.39497405626220405No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG463230.33048034261571946No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC427390.30491115348861764No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG400630.2858198727676008No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG387250.2762742324070923No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC362680.25874535470472365No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA352510.25148981191949416No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA340140.24266473185525728No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT327930.2339537999567664No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT309330.22068407568879503No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT297570.21229418550646473No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT288320.20569499467427466No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA274340.19572129869221874No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC270120.19271064082066824No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC239900.1711509060153943No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA238380.1700664984408074No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT238180.1699238132336249No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG236420.16866818341041903No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG234170.16706297482961605No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT232830.16610698394149337No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC207140.14777906907890279No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA201770.14394797126605297No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC201120.14348424434270987No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC191630.13671383126190084No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT191290.1364712664096906No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA182640.130300131199048No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG182480.130185983033302No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA175330.12508498687652808No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA172670.12318727362100099No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT169950.12124675480331913No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC161110.11494006864585318No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC147670.10535162272318997No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT145330.10368220579915488No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC141910.10124228875633434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT55250.064.386911
CGCAAAA97950.058.86571
CACCCGG132150.058.6939624
GACCGTT61450.057.81332
ACCGTTG62850.057.35883
CCAGTAG88400.053.4625
ACCCGGC146850.052.7215355
GTAGCAT107000.044.258978
TAGCATA109950.043.4175579
TCACCCG183650.043.3716243
CGGCCCG184250.042.3822678
TTAACCC135850.042.2723668
CAGTAGC115100.040.937386
TAACCCC143550.040.733289
GCCAGTA116350.040.6599964
CTCACCC196750.039.68582
TGCCAGT120350.039.623353
GTTGACT170250.038.553961
CAAAATT158350.036.4136473
CGGACAC129800.035.62850612-13
Produced by FastQC (version 0.11.5)