FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14149362
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA669760.47334996447189637No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA354930.25084523245641743No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG323830.22886544283763469No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT224090.1583746320152103No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC215810.15252277805882697No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG197820.13980842387098444No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA186520.13182219806094436No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG184390.13031682983303416No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC182760.12916483442857704No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA175200.1238218373379662No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG173240.12243661587003005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG50800.038.8712921
CCCGTAT87550.037.7545621
GTATCGA90200.037.0643124
ACGACTT30350.035.988951
TATCGAA96000.034.726075
TGTCGAG14000.032.563645
GTTACGA11150.028.9616874
GTCGAGG16700.027.2988626
TGTTACG12550.026.1092973
CGTATCG177750.025.247133
CCGTATC173600.025.2215062
TTCGCGC41400.025.1208087
ATCGAAG156550.024.9652866
GTCCGAT5100.024.316191
CGCTAGG5900.024.2527851
GAAGGCC141950.024.0872339
TTACGAC13700.023.9176395
CGCGTAT1601.7961447E-623.848571
TCGACCG37800.023.61902630-31
CTTCGCG44700.023.5849696
Produced by FastQC (version 0.11.5)