FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19932469
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1152170.5780367700559323No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC675510.33889931046675653No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA543230.27253522882689546No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC415890.20864951552163458No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT335270.1682029456561553No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC308800.15492310561225506No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC291170.14607824048290255No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA286750.1438607530256287No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC275490.1382116786435238No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC266410.13365629717021008No Hit
AGACAGGGTGTTGGCTTTACACCAACGAAAAAGAAAAACAGATGAATAAG261220.1310525053368953No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC228340.11455680678595312No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA226250.11350826633669919No Hit
GACAGGGTGTTGGCTTTACACCAACGAAAAAGAAAAACAGATGAATAAGC224810.11278582698410317No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG219050.10989606957371914No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC218800.10977064607500454No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGAGCTGTGACGATAA206960.10383058917588182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA17350.046.710991
TCGGTAT17800.039.9132352
CGCTAGG10000.032.1253781
GTTACGA16200.029.2950331
CCGGTAA18450.026.9096571
GTCCCGT28850.026.5729081
CCCGTCG41750.025.70731
ACCCGGG968400.025.331018145
TTACGAC19000.025.1826362
AACCCGC13950.025.1224881
ACGACTT38750.024.8712621
CGGTAAT17700.024.5747362
GAACTCT1080450.024.4526129
TACGACT20200.023.325293
CTTGAAC1108600.023.1007586
GTCCTCT131100.022.8337251
TCTTGAA1068300.022.7712155
CCTCTTG1008750.022.7362063
CTCTTGA1051650.022.3943334
TTGAACT1207250.022.2158577
Produced by FastQC (version 0.11.5)