FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19662030
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT819600.4168440389929219Illumina Single End PCR Primer 1 (100% over 50bp)
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA754390.38367859269871935No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG475610.2418926224809951No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG447220.22745362508347308No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC430630.21901604259580523No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT403540.20523821802733494No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT334530.17014011269436574No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG326710.16616290383037763No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC314270.15983598845083646No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG292660.14884526165406115No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC285660.1452851002668595No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT282430.14364234008390792No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC278450.14161813403804185No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT273300.13899887244602924No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA244080.12413774162688186No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA225340.11460668099885923No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT224660.11426083674981678No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA223310.11357423419657074No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA222230.11302495215397391No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG206260.10490269824631536No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT202430.10295478137303218No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT197640.1005186137952185No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT63000.067.5227361
GACCGTT77850.054.670992
ACCGTTG80000.053.1888543
CGCAAAA100800.052.62371
GTTGACT246500.048.6806871
TTGACTA258400.045.5410962
CTACCGT152100.043.1718678
ACTACCG153900.042.7611167
CCGTTGA100850.042.2617454
GAGTCTA115200.041.7290532
GTCTAAC116900.040.923754
GTAGGTC100300.040.6991122
TGACTAT335850.035.3973733
TCACATG209000.035.212532
AGTAGGT120000.034.524671
AACACGT139100.034.3889278
GACTATT345000.034.183344
ATCACAT222100.033.0767971
CGTTGAC132400.032.244425
TAGGTCT132250.031.5717963
Produced by FastQC (version 0.11.5)