FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18087447
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA743980.41132394195819894No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG437210.24172012777701574No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG430760.23815411871006448No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC412550.22808636287918355No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT346470.1915527382056738No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG277470.15340473423363726No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG255490.1412526599248639No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC241260.13338532519265986No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT238170.13167695805825996No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT235470.1301842100767455No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC234570.12968662741624065No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA233300.12898448299530607No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT228130.12612614704551725No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA212380.11741845048668284No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA211960.11718624524511391No Hit
CCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTAC209770.11597546077121886No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT208690.11537836157861306No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT207560.1147536189048681No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT207050.11447165539724871No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT206160.11397960143297171No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA203730.11263612824960868No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA199060.11005422711121145No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC195440.10805283907673648No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC186560.10314335682642221No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT186100.10288903679994198No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT185890.1027729341791575No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT51050.058.40721
GTTGACT211050.054.0925751
GACCGTT62200.048.145942
TTGACTA237250.047.7391622
ACCGTTG62950.047.6749763
GTAGGTC82250.045.754252
CTACCGT124100.045.0294348
CGCAAAA88300.044.5767141
ACTACCG131750.042.3595777
TGACTAT292000.039.3737033
AGTAGGT98250.038.2550051
GACTATT306650.037.3729024
CCGTTGA85300.035.7767564
GTCTAAC80700.035.4806444
ATCACAT187850.035.0932541
CCGTCAT119850.032.7186161
TCACATG203600.032.4088482
GAGTCTA87850.032.1075972
TAGGTCT115600.032.1068763
TACTCTA121950.032.0949489
Produced by FastQC (version 0.11.5)