FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16316420
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC574260.3519522052018764No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA491760.30138964307121296No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA464000.2843761070136709No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC452210.2771502572255434No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG402830.2468862654920626No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA372040.228015704425358No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG350220.21464267284122376No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT332360.20369664423936135No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC331210.20299183276723692No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA311800.19109584087685902No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG296880.18195167812547114No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT284730.17450519170259163No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG262170.16067862925813384No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT259270.1589012785892984No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG238820.14636789197630362No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT228290.13991427040980803No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA223060.13670891041049446No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT219820.13472318069772657No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA214030.13117460815546547No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT212460.13021238727613044No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG193690.11870863829197827No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT188180.11533167202119092No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG178460.1093744828828873No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA174580.10699651026389369No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC171640.10519464441341912No Hit
AGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGG166930.10230798177541396No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT163910.1004570855616612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT37800.062.2885131
GAGTCTA178350.055.8209952
ACCGTTG47850.049.0848433
GACCGTT48350.048.740172
GTTGACT162100.047.7315941
GGAGTCT216850.047.016161
GTCTAAC216450.046.04734
AGTCTAA219700.045.2367863
GTAGGTC62700.044.7552072
TTGACTA171800.044.1477622
CTACCGT111400.042.8742688
CGCAAAA78850.041.9738161
AACACGT244200.040.6603748
ACACGTG258550.038.1243869
ACTACCG127200.038.0045177
TCACCCG102100.037.9850233
TAACACG269000.037.1003657
AGTAGGT77100.036.6844141
AGATCAG171800.035.5798872
AGACGTG242500.035.2719354
Produced by FastQC (version 0.11.5)