FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004828055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34779265
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT9365952.6929695035245858Illumina Single End PCR Primer 1 (100% over 50bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA778580.2238632702559988No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG723820.20811825666816136No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA700430.2013929851594046No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG655040.18834210556203532No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC593190.17055852100382224No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG583420.1677493759571975No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT548960.15784117346930707No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA520760.14973289401026732No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC494300.14212491264550875No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT492620.14164186620965108No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT478940.1377084880890956No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG476340.13696091622407777No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG473470.1361357118961542No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG466270.13406551288533555No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC433710.12470361291418897No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA427130.12281168104041301No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA391660.11261307563572721No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA388410.1116786108044549No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT387710.11147734145618086No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT378730.10889534324546536No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT363720.10457955336318925No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG348100.10008837162027433No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)