FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17414809
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC692560.3976845224084858No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA622460.35743142517382764No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA579480.3327512808208232No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG579170.3325732714036657No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC574030.3296217604224083No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT559990.3215596564969504No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA505280.290143865488275No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT443670.25476592938802833No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG433470.24890884533961874No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA413160.2372463573961678No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG402480.2311136458631272No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC400900.23020637205955No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG394730.2266634104341885No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT357100.2050553640869676No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT345010.19811299681782327No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG329040.18894264071457806No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG323670.18585905823026827No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC315480.18115616427375117No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA292210.16779397350840886No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT278590.15997304363200307No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA276380.1587040087548477No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA274330.1575268497059026No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA265810.15263446185370164No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT265710.15257703946107018No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA250600.14390051593445555No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT242240.13910000391046493No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA237330.13628056443225994No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG234370.1345808616103685No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG228400.13115274477026995No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA223300.12822420274606514No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT223040.12807490452522333No Hit
AGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGG213690.12270591081418118No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT209950.12055831332976434No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT197760.11355852366798855No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT194420.11164061575409756No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC189730.10894750553968177No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC187850.1078679645582102No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT186790.10725928719631664No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA184650.10603044799400325No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG179920.10331436882253489No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA175320.10067293876148743No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT44750.064.9694441
CCAGTAG102550.060.147065
TCACCCG146250.059.778743
CACCCGG147150.058.227934
ACCGTTG51400.056.4146923
GAGTCTA185550.055.491632
GTTGACT175800.055.2529531
TAGCATA113700.054.8218239
GACCGTT53200.054.6553732
GTAGCAT113500.054.5327728
CGCAAAA79650.053.3133241
GTAGGTC68800.051.01022
AGATCAG177800.049.3869822
CTACCGT126300.046.8254938
ACCCGGC189900.045.2668465
GGAGTCT228650.045.2643361
AGTCTAA228650.045.1792453
CAGTAGC138450.044.9697766
TTGACTA213500.044.8307652
GTCTAAC236900.043.4813654
Produced by FastQC (version 0.11.5)