FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18039959
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT922460.511342625556965No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC773620.42883689480669No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA657630.3645407398098854No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC646720.3584930542247906No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG618430.3428112004023956No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG541620.3002334983133831No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA519310.2878665079006No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT512940.2843354577468829No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA491090.2722234568271469No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT416120.23066571271032268No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA404560.22425771588505272No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA382960.21228429621153794No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA340160.18855918685846237No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG339850.18838734611314803No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG332370.18424099522620865No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG323740.17945717060665162No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA311650.17275538153939263No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT300810.16674649870323985No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA295400.1637476005350123No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACC264010.14634733925947394No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG263320.1459648550199033No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA262350.1454271597845649No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG260200.1442353610670623No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC240050.13306571262163067No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT217880.12077632770673148No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT217720.12068763570914989No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT215230.11930736649678637No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT210020.11641933332553582No Hit
AGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGG206550.11449582562798508No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG205120.11370314089959961No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC202200.11208451194373557No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA196090.10869758628608855No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT195230.10822086679908752No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC193590.10731177382387622No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT191820.10633061860062987No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT186370.10330954743300691No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC186000.1031044471885995No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC184680.10237273820855135No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG183690.10182395647351528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT36200.079.477151
TCACCCG183800.078.2074363
CCAGTAG128300.072.70335
CACCCGG201550.071.028634
ACCGTTG42750.067.995333
TAGCATA142750.065.749119
GTAGCAT146100.064.437988
GAGTCTA217300.060.630352
GGAGTCT220300.060.2630651
ACCCGGC240000.059.582415
GACCGTT50350.057.7483752
AGTCTAA230800.057.161873
CAGTAGC176300.052.9499786
CGCAAAA69250.049.5563661
GTCTAAC270300.048.7525144
CTCACCC298850.048.040422
GTAGGTC48800.047.9929662
AGATCAG178700.047.3117372
TGCCAGT206600.045.5072443
AGTTACG218250.045.435642145
Produced by FastQC (version 0.11.5)