FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17896095
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA857290.4790374659946765No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC541860.3027811374492592No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG533300.2979979710657549No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG523400.2924660379820291No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC521790.29156640037952414No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC460600.2573745836731421No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA446230.24934489898494616No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA414130.2314080250468049No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT397130.22190874601414443No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG373970.20896737528494344No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC356360.19912723976934632No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA346010.1933438551818148No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT341780.19098021104604104No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT324550.18135241235587987No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG322770.18035778196304836No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA303510.16959565760016362No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT299970.16761757243689196No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT285570.15957112431510898No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA276670.15459797235095143No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG243540.13608555385965487No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA238100.1330457845692035No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG236810.13232495692496044No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT230250.12865935278059262No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG228070.12744120993993382No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG228010.12740768307275974No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT225840.1261951280432966No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT222950.12458025060774433No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG215600.12047320937891759No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC201640.11267262494974462No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA200270.1119070948159361No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC199140.11127567215082396No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT195250.10910201359570341No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT192490.10755977770569503No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA183710.10265367947588566No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC181050.10116732169783407No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA180660.10094939706120246No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA179430.10026209628413349No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT69700.074.9592741
ACCGTTG83700.061.7730833
GACCGTT90350.057.1981282
GAGTCTA169700.054.4473232
GTTGACT259800.053.028491
GTCTAAC188150.049.0094344
CCGTTGA107300.048.5764734
TTGACTA280850.048.121072
GGAGTCT203300.047.133551
CGCAAAA107600.046.76561
GTAGGTC77000.045.4345552
AGTCTAA207550.044.617313
AACACGT209650.044.014818
CTACCGT147000.043.2954678
ATCACAT187450.041.8282051
ACTACCG155350.041.2014277
TCACATG192300.040.8392682
ACACGTG227900.040.3955159
TGACTAT341800.039.5041583
GACTATT345050.039.308694
Produced by FastQC (version 0.11.5)