FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18047621
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1039630.5760482226438598No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA588110.3258656639564849No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC381470.21136857871738332No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT362860.20105697033420636No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC356440.19749971478235273No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG290500.16096304327312724No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC267760.1483630446361878No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC245810.13620077682260726No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA234690.13003929991659288No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC231600.12832716289864463No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA222940.12352874653119102No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG214240.11870816657774451No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC203450.11272953925617123No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC194000.10749339206535866No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG190630.1056261099454604No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC187310.10378653230805324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA121600.036.3062024
CCCGTAT128150.034.775251
CGTATCG155850.033.448063
TATCGAA140600.031.6055835
CCGTATC166600.031.2122732
ACCCGGG724750.029.91197145
ACGACTT36700.028.373141
TCGGTAT19250.028.2560712
GTCGGTA22400.027.3066671
GAACTCT887350.025.4557069
ATCGAAG194300.025.2952826
CCTCTTG851900.024.0169143
CTTGAAC926950.023.9068166
TTGAACT962750.023.7933857
CTCTTGA888050.023.4936494
TCTTGAA920200.023.3420015
GTCCGAT13750.023.3016871
CCCGTCG39700.021.4601521
AGGAACG50350.021.3081077
GTTACGA15650.021.3078964
Produced by FastQC (version 0.11.5)