FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18047621
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA873490.48399176822252643No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC570420.3160638180511437No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG545210.3020952179791453No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG540910.29971263248491314No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC530100.2937229233703434No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC493620.2735097329448574No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA467960.2592917925304393No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA418320.2317867823133032No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT409140.22670023932794245No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG394330.218494171614087No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA364150.20177174598247605No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC359460.1991730655248135No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT343800.19049602160860982No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT333510.1847944391119472No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG331390.18361976905432578No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA326200.18074404377175252No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT308140.17073718469597737No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT308020.17067069393799883No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA290500.16096304327312724No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG255540.14159206911536984No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG245830.1362118586156037No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA244490.13546937848484297No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT236810.13121396997421433No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT234630.1300060545376036No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG234390.12987307302164644No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG233830.12956278281774644No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG231340.12818309958969107No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT226990.12577280961296783No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA215080.11917360188359452No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT208320.11542795585080161No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC207420.11492927516596231No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC205870.11407043620873909No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT194510.10777597778676759No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA186180.103160411003755No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC185630.10285566169635323No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG184650.10231265383952821Illumina Single End PCR Primer 1 (100% over 50bp)
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA182420.10107703392042641No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA182280.1009994613694514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT72550.077.539751
ACCGTTG85250.066.068373
GACCGTT91550.061.460412
GTTGACT256100.052.8341561
GAGTCTA172400.052.657372
CCGTTGA112650.049.7391554
GTCTAAC191500.047.2763334
TTGACTA282400.047.2438432
ATCACAT193500.046.0950431
GGAGTCT205400.045.7668951
TCACATG198300.044.9753462
GTAGGTC83600.044.927852
CGCAAAA112250.044.181111
AGTCTAA207750.043.6153
AACACGT215200.042.4358
CTACCGT160400.040.7114268
CGTTGAC139900.040.047645
ACTACCG165550.039.2698487
TGACTAT345400.038.9513553
ACACGTG234800.038.8322839
Produced by FastQC (version 0.11.5)