FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16996891
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC554940.3264950042922556No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA505770.2975661843098247No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA472890.27822147003237235No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG411360.24202073190914739Illumina Single End PCR Primer 1 (100% over 50bp)
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA320110.18833444304608413No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC318870.18760489786043813No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG302260.17783252243013148No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG292450.17206087866304492No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG288770.1698957768217729No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC288460.16971339052536139No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG281940.16587739487180334No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT281010.1653302359825688No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA269980.1588408138876692No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA257540.15152182831554312No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG236800.13931959674272196No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT234740.13810761038592292No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA224190.13190059287901534No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT213840.12581124395043775No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT188760.11105560422785556No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT182670.107472596017707No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG179250.10546046332826396No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG177690.10454264841728995No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC172730.10162446767470591No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT42650.063.536461
GAGTCTA184000.053.4058152
AGTCTAA205100.047.8019683
ACCGTTG57700.047.1292153
GTCTAAC209300.046.9328584
GACCGTT58150.046.552912
GTTGACT179300.044.638171
GGAGTCT232800.043.7937281
TTGACTA183000.043.108822
AACACGT236900.041.6463558
TCACCCG109850.041.3906483
ACACGTG256000.038.539269
TAACACG257750.038.417887
CTACCGT94500.037.2043658
AGTTACG78400.036.88969145
GACTATT224950.035.4497874
CACCCGG126700.035.0744444
TCTAACA281550.035.011125
GTAGGTC52400.034.625532
TGACTAT232250.034.28323
Produced by FastQC (version 0.11.5)