FastQCFastQC Report
Sat 11 May 2024
EGAF00004828077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17218959
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC577000.3350957511426794No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA512300.2975208896194015No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA488630.2837744140049349No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG437560.2541152458752007Illumina Single End PCR Primer 1 (100% over 50bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC344550.20009920460348388No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA334930.1945123395671016No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG310860.18053356187212014No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG307050.17832088455521614No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG303280.17613143744636361No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG297130.17255979295844773No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC294200.1708581802186764No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT284420.1651783943500882No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA277380.16108987773302672No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA276660.16067173398809997No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG248310.14420732403160957No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT247240.14358591596623232No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA235910.13700595953564906No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT217760.12646525263228747No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT198430.11523925459140706No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG188980.10975111793924361No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT186210.10814242603167822No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG184440.10711448932539999No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC174220.10117917116824543No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)