FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004828079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23087610
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA820460.3553680957015473No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC649880.28148431128211193No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG545090.23609633045603245No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC530990.22998915868727857No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG514660.22291610088701255No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG487660.21122151664897318Illumina Single End PCR Primer 1 (100% over 50bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA482160.20883928652640962No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC473390.20504071231279458No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG442300.19157461512906707No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT405430.17560501065289996No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA383050.16591149971781402No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT374340.16213891346917245No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA367140.15902035767236192No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG311240.13480823697212488No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG305240.13220944047478278No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG282040.12216076068506009No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT281340.12185756776037016No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC270510.11716674008266771No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA269180.11659067352575687No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT262530.11371034074120275No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG259750.11250623169743425No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT253140.10964322422286239No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT243090.1052902400898144No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT242680.10511265566249604No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA242240.10492207725269094No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG238620.10335413669929457No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)