FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004828081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23258082
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA836600.35970291961306183No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC680410.2925477689862818No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG559560.2405873364794225No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC540410.23235363947895618No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG522340.22458429719183207No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG516230.2219572533969052Illumina Single End PCR Primer 1 (100% over 50bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC511500.21992355173569342No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA500350.21512951927850285No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG471910.20290151182715757No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT412630.17741359756148423No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA407630.17526380722193688No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT395770.1701645045365306No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA393150.16903801439860777No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG337860.1452656328238932No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG308690.132723755982974No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG304040.13072445096719498No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT291330.12525968392406564No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC276800.11901239319734103No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT276590.11892210200308004No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT273980.11779991144583632No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA273820.11773111815497081No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG259970.11177619891442467No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA258000.11092918152064302No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT253270.10889547985943122No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG246520.10599326290104231No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT245880.10571808973758026No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA236110.10151739941410473No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)