FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21217695
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA876620.4131551518673447No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA348570.16428268951929037No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT342660.16149727856866639No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC293560.13835621635620646No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC260610.12282672552320127No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT238430.11237318662559717No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC228490.10768841761557982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGG595550.030.587969145
GAACTCT656750.028.8602359
CCCGTAT43950.028.5727621
GTATCGA31700.028.3533924
CTCTTGA653300.026.8389684
TTGAACT735700.026.6210487
CTTAACG218400.026.352373
AACGGTT217900.026.1460366
CTTGAAC716550.026.0983926
CCTCTTG673450.025.4337373
TTAACGG232150.024.7285754
TCTTGAA741350.024.628985
CGTATCG67550.024.573213
TAACGGT243800.023.6955175
GTCGGTA21950.022.9507681
CCCTCTT694800.022.7664242
TCTTAAC257300.022.6525022
GCCCTCT650150.022.4369891
CCGTATC79650.022.3897482
ACCCGTT127950.022.3798262
Produced by FastQC (version 0.11.5)