FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16881308
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1235690.731987118533706TruSeq Adapter, Index 1 (100% over 50bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA852700.5051148880169712No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA442590.26217755164469486No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT377360.22353718088669433No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC321630.1905243361474123No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC317060.18781719994682877No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC259280.15358999432982326No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA230780.1367074162736679No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC223420.13234756453706076No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC221880.1314353129508685No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA211240.12513248380990383No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC198240.11743165873165752No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT181870.10773454284466583No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA52400.033.477034
CCCGTAT59750.032.8663181
ACGACTT43150.029.4377841
ACCCGGG630750.028.249239145
TATCGAA64100.027.5925675
GAACTCT691300.026.9357789
GTTACGA19500.026.7645994
CCTCTTG646600.026.703943
CGTATCG96550.026.6529543
AACGGTT262850.026.198476
CTTAACG272750.025.5138363
CTCTTGA691000.025.3758534
TACGACT20900.025.3184366
CTTGAAC724550.025.2911746
TTACGAC20800.025.0916635
TTAACGG277800.025.0235674
TTGAACT758000.024.9880167
TCTTGAA722250.024.8799345
TAACGGT279750.024.8489935
TCTTAAC289600.024.3323082
Produced by FastQC (version 0.11.5)