FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16881308
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1299600.7698455593606846Illumina Single End PCR Primer 1 (100% over 50bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA716980.4247182742000798No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC569420.3373079858503855No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC442580.2621716279330962No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA397220.23530167212161524No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG395640.23436572568902836No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT338610.20058279844192167No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA331000.19607485391534826No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT318230.18851027420387093No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT305290.18084499139521654No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT285340.16902718675590778No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT257850.15274290357121617No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG253700.150284563257776No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT244340.14473996920143867No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA243230.1440824372139884No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG239320.14176626597891584No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC238390.1412153608002413No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC231890.13736494826111814No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT228850.13556413993512823No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC218100.1291961499665784No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG212600.12593810858732038No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA207570.1229584816531989No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG203320.12044090422377222No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG192090.11378857609848717No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG190230.11268676574113806No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC189610.1123194956220217No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA187670.11117029557188342No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT186170.11028173883208575No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT185260.10974268107660852No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT178890.10596927678826783No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC174710.10349316534003171No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA169530.10042468273193049No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT36600.078.935331
ACCGTTG47900.059.6447753
GACCGTT48800.058.598612
GAGTCTA178150.056.9143072
CGCAAAA79850.055.1988451
AGTCTAA194300.052.173033
GTCTAAC238650.049.7887081
GGAGTCT209000.049.4442561
TCACCCG105500.047.83753
ACGTGCG271550.043.406278
CTACCGT91600.043.051198
AACACGT277300.042.6826325
AGTTACG85300.041.724125145
GTAGGTC56050.041.307222
TAACACG285550.041.279124
ACACGTG288400.041.0454446
CACCCGG124850.040.528354
CGTGCGC296650.039.7833029
ACCCGGC129650.039.3563655
TCTAACA301750.039.229932
Produced by FastQC (version 0.11.5)