FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17772909
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA817640.4600484929056915No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA330600.18601344326919134No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT297310.16728268850079636No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC248300.13970701138457411No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC245350.1380471818091231No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC210840.11862998904681277No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC202830.11412312975889315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT65200.031.8059921
GTATCGA58200.031.2607334
ACCCGGG542400.030.431599145
GAACTCT602550.028.6050539
GTCGGTA19150.028.2163681
ACGACTT42600.027.5965271
CTTGAAC630100.027.0797966
CCTCTTG584700.026.9514543
TTGAACT656200.026.853277
TATCGAA68650.026.818945
CGTATCG99500.026.0804863
AACGGTT202900.025.8288296
TCTTGAA649350.025.4509755
GCCCTCT537250.024.5731141
CTCTTGA666450.024.3846474
CTTAACG217500.024.3620513
GTTACGA17400.024.3397961
CCGTATC109500.024.098472
CCCTCTT602150.023.7412972
TAACGGT224700.023.7100625
Produced by FastQC (version 0.11.5)