FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17772909
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA713230.40130177901659203No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC568060.31962128428159964No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG350370.1971371146951802No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA338580.19050342293430975No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC315460.17749486029552056No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG310530.1747209756151905No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA310430.17466471020585317No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT298800.16812104309992246No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC278230.15654724839923503No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA272430.15328385465767028No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT263430.14821996781731117No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC248350.13973514408924279No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT242100.13621855600566007No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT225090.12664780987738133No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT223710.12587134722852628No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG223190.1255787670999722No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG214260.12055426604614923No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA197510.11112980998214754No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG192190.10813649020540193No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG191860.10795081435458877No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG189870.10683113270877603No Hit
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC181680.10222299568404924No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT181400.10206545253790474No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC180270.10142965341239298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA163700.056.9312672
CGACCGT31500.056.00761
GTCTAAC224500.052.566281
AGTCTAA178500.052.244693
GGAGTCT204450.047.3155561
AACACGT263000.044.892875
ACGTGCG264850.044.2852448
TAACACG269500.043.5508544
ACCGTTG40800.043.3679663
ACACGTG277750.042.5149236
TCTAACA282450.041.501662
TTGACTA126900.041.2961962
AGTTACG77650.040.980564145
CGTGCGC291100.040.3421869
GACCGTT43950.039.9660262
TCAGACG205700.038.9202582
TCACCCG113900.038.6459853
CACGTGC316050.037.156617
GTTGACT150200.036.8664441
CTACCGT76300.036.577738
Produced by FastQC (version 0.11.5)