FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18288908
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA757230.4140378419531664No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC604060.3302876257018735No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG352460.1927179031137343No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA350200.19148218144024784No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC341520.18673613536685732No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA330330.1806176727445947No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG328810.1797865679022498No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT322090.176112209651883No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC277540.15175318285815645No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA276480.1511735965865212No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT268630.14688137749941113No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT259520.14190021624035729No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC254690.1392592712479061No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT236980.12957580627558518No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT231740.12671068168750152No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG230160.12584677007506406No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG226410.12379634694427902No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA215740.11796220966281859No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG204970.11207339443120387No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG203140.11107278794338077No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG202950.11096889983808766No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT196640.10751872118335332No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC191260.10457704746505368No Hit
GGCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGC185150.10123622471062788No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT184920.10111046542527306No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA184520.100891753624656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT32100.058.1187321
GAGTCTA172600.057.049742
AGTCTAA186050.052.9889343
GTCTAAC234600.051.563771
GGAGTCT209750.048.2841641
AACACGT272400.044.5417485
ACGTGCG275950.043.6251378
TAACACG280350.043.282334
ACCGTTG43300.043.0250243
ACACGTG288050.041.819816
TTGACTA134300.041.464832
AGTTACG78500.040.907715145
TCTAACA301150.040.4983982
GACCGTT46400.040.474032
TCACCCG115600.039.8191343
CGTGCGC306950.039.314269
TCAGACG220900.038.174972
GTTGACT154100.037.5205351
CACGTGC330400.036.43577
CTACCGT80150.035.2661978
Produced by FastQC (version 0.11.5)