FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20380186
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA779760.3826069104570488No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT338550.16611722778192506No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC304580.1494490776482609No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA296880.1456708981949429No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC277850.13633339754602827No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC227540.1116476562088295No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC214620.10530816548975559No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA26950.037.38544
ACGACTT62400.036.508171
TTACGAC29000.034.7423825
TACGACT31350.031.675676
TGTTACG31900.031.584763
GTATCGA53600.029.7511564
CCCGTAT65600.028.4941831
ACCCGGG563500.027.813095145
GAACTCT610700.027.0245659
CCTCTTG563700.026.9137783
TTGAACT641000.026.3362677
TGTCGAG26750.026.2839585
CTTGAAC633200.025.6647766
AACGGTT178750.025.5873976
CTTAACG182600.025.3263933
GCCCTCT500200.024.6842541
CTCTTGA630850.024.634554
TCTTGAA661750.024.1741495
TATCGAA69200.023.77735
TAACGGT199600.023.168775
Produced by FastQC (version 0.11.5)