FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19289294
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA772110.40027903561426353No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT353070.18303935851669845No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA294950.15290865492536948No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC280910.14563000595045106No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC244730.1268734874381613No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC227000.11768186020701431No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC205820.10670167606963737No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT201430.10442580220924623No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA199440.10339414184884113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT55100.037.0768361
GTATCGA55950.034.721574
CCCGTAT65850.033.018451
ACCCGGG532900.028.877916145
GTTACGA19950.028.7044224
CTCTTGA560700.027.0714024
GAACTCT598150.026.9268919
AACGGTT167600.026.9010126
CTTAACG171300.026.6646333
CCTCTTG558350.026.580543
CGTATCG105050.025.950413
CTTGAAC620950.025.9265166
TTGAACT639700.025.8351367
GCCCTCT499700.024.748941
TTACGAC23150.024.7351675
TCTTGAA646150.024.5556355
TTAACGG188550.024.1049184
CCGTATC113500.023.5841082
CCCTCTT574900.023.4319572
TAACGGT196650.023.3318165
Produced by FastQC (version 0.11.5)