FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19289294
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA849350.440321973422148No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC533960.27681676685523066No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT524080.2716947546136214No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT478070.24784214497430543No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT380830.1974307613332038No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA332900.17258278089389897No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG320300.1660506600189722No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC307390.15935782823363054No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT281970.14617953357961155No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG280130.1452256365629556No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA277670.1439503177254699No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC271780.14089681042758745No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT233160.1208753415236452No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT231990.12026878744240199No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG231660.1200977080861539No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC212250.11003513140501668No Hit
GACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGG211850.10982776248835235No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT210050.10889460236336279No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT200080.10372593211550407No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT197530.10240395527176889No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT195330.10126342623011501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT36250.074.777531
GTCTAAC231800.064.125681
TCACCCG148050.057.920783
ACCGTTG49000.056.9538043
ACCCGGC153800.055.23395
AACACGT272400.054.5216875
ACGTGCG277150.053.3625348
GACCGTT52150.052.8311232
GAGTCTA160600.052.639722
TAACACG283300.052.3244674
ACACGTG286600.051.8399776
AGTTACG127900.051.34229145
CGTGCGC297000.050.0662469
TCTAACA305450.048.5890352
AGTCTAA177150.047.5469973
CACCCGG182050.047.1432044
CCAGTAG121050.044.9686245
GGAGTCT193850.044.6246721
CACGTGC346050.042.8809177
TCAGACG202350.040.0947462
Produced by FastQC (version 0.11.5)