FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21827634
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1688970.7737760308790224No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC912130.4178785478994196TruSeq Adapter, Index 6 (100% over 50bp)
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA890570.40800116036396794No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC689300.31579235752257895No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT454960.208433034931775No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC415790.19048789254941695No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC404170.18516436550108914No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC382860.1754015116801024No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT371000.16996803226588827No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC356880.16349916807291162No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT332720.1524306299070252No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG318320.14583348795384787No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA310690.1423379189883796No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC307280.1407756791230786No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA305350.13989147884740966No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC300040.13745878275217552No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT280610.12855722246396473No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG265700.12172643173327903No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA260690.11943117609540274No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT245770.11259580401613845No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC242350.11102898280225881No Hit
CCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTC231510.10606280094306143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT113700.052.0391461
GTTACGA44350.047.377121
TTACGAC46250.045.6450542
GTCGGTA19300.044.228111
TACGACT49650.042.6402053
TCGGTAT18200.039.063142
TGTCGAG34150.037.7804875
GTATCGA138600.035.7209974
CCCGTAT142750.035.520451
TATCGAA143650.034.5134775
TTACTTC219050.031.5022018
ACTTTTA218500.031.251124
TACTTCC223550.030.9653439
TGTTACG43750.029.16693
GTCGAGG45500.028.9939386
CCCGTCG128200.027.9992831
GGACCCG156950.027.379391
GTCCCGT31900.026.5299931
ACCCGGG1238200.026.11637145
CGTATCG289800.025.8438743
Produced by FastQC (version 0.11.5)