FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18922505
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1404150.7420529152984766No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG837580.44263695530797853No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC727510.3844681240670831No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA721560.3813237200888572No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC703600.37183237631592647No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG695910.36776843235079076No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT658910.3482149958475371No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA603380.31886898695495125No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA586050.30971058007383273No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA583890.30856908215904816No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA567060.299674910906352No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG477670.2524348652570048No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT476780.25196452583841306No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA448890.23722546248501455No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG447500.2364908874380004No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC405060.21406256729751164No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA367030.19396480539970792No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG318430.16828110231705581No Hit
AAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTG317570.16782661703616936No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT311200.16446025513006868No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA295330.1560734162839434No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT279040.14746461950994333No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA271640.14355393220929258No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG254990.13475488578282843No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC250900.13259343834233364No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC246750.13040028262642817No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA245670.12982953366903588No Hit
GACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCA241440.12759410025258283No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT230480.12180205527756499No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT226500.11969873967532313No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT219820.11616855167960057No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT219510.11600472558997871No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT210070.1110159569253648No Hit
AGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAA202890.1072215333012199No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG202010.10675647859519656No Hit
GCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAAC201120.10628613917660477No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA199120.1052291966629154No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG196480.1038340325448454No Hit
AGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGG196140.10365435231751823No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG192810.10189454303222538No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG284050.081.231653
CACCCGG289100.079.411924
CCAGTAG173100.077.5142065
TAGCATA182850.073.273269
GTAGCAT190500.070.710258
ACCCGGC323750.070.664155
CAGTAGC202600.066.285836
GCCAGTA208900.064.336434
GAGTCTA205400.059.5158162
GTTGACT168900.057.4174841
TGCCAGT243300.055.269463
AGTCTAA226450.053.8759843
CTCACCC438050.052.834122
GGAGTCT236600.052.824391
CGACCGT36450.052.235981
AGTTACG341600.049.819893145
GTAGGTC69950.049.546782
ATCACAT147200.049.0213551
TCACATG148600.048.451332
TTGACTA194800.048.3126642
Produced by FastQC (version 0.11.5)