FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19941818
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG4881842.448041597812195TruSeq Adapter, Index 13 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1008690.5058164706948985No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA940500.4716219955472465No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC797780.40005379649939643No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT631090.3164656301647122No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT511050.2562705165597239No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC419800.21051240162757479No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT393240.19719365606485828No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC354420.17772702569043605No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC349830.17542532982699974No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG332520.16674507810672026No Hit
AGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGA323990.16246763459580266No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT319920.16042669730513037No Hit
CCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACT301050.1509641698665588No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC295800.1483315111992297No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA278070.1394406467855639No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA277800.1393052529112441No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG268810.13479713835518908No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT268610.1346968465964337No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG268180.1344812193151096No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA248660.124692743660583No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG248390.12455734978626322No Hit
TTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGG245930.12332376115357187No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC240910.12080643800881144No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC232730.11670450507571577No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT231440.11605762323174346No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA230750.11571161666403736No Hit
TCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAA225400.11302881211733053No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT225160.11290846200682406No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC223750.11220140510759852No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA219500.11007020523404636No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTATG218620.10962892149552263TruSeq Adapter, Index 13 (97% over 39bp)
CGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC209320.10496535471339674No Hit
TCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG208710.10465946484919278No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC202850.1017209163176597No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA201840.10121444293594496No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT200560.10057257567991043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG54500.050.1535073
GTTACGA70500.047.3904651
TTACGAC71850.045.4318122
TACGACT77950.042.0415533
AGCACAC1966550.040.993879
TGTCGAG37100.040.2462655
GAGCACA2050700.039.4141968
ACGACTT132200.037.136271
AGAGCAC2222300.036.41647
CGGAAGA2258850.035.817423
AAGAGCA2261700.035.756376
TCGGAAG2320500.034.936482
GTCGAGG42900.034.4678236
GAAGAGC2453100.033.0456435
GGAAGAG2603450.031.1864534
ATCGGAA2688550.030.3264641
CTTTAAG320650.029.4158841
TCTTAAC507200.029.0329482
AACGGTT507400.028.7421026
GTCGGTA12750.028.0349981
Produced by FastQC (version 0.11.5)